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msrB [2019-08-05 12:53:41]
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msrB [2019-08-05 12:53:41]

peptide methionine sulfoxide reductase
locus
BSU_21680
pI
5.84
mw
16.46 kDa
protein length
143 aa Sequence Blast
gene length
432 bp Sequence Blast
function
regeneration of methionine and restoration of protein function after oxidative damage
product
peptide methionine sulfoxide reductase
ec
1.8.4.12
synonyms
yppQ
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

    • Gene

    Coordinates
    2,287,097 → 2,287,528

    The protein

    Catalyzed reaction/ biological activity

  • Peptide-L-methionine + thioredoxin disulfide + H2O = peptide-L-methionine (R)-S-oxide + thioredoxin (according to Swiss-Prot)

    • Protein family

  • MsrB Met sulfoxide reductase family (single member, according to UniProt)

    • Structure

  • [PDB|1XM0]

    • Expression and Regulation

    Operons

    genes
    [gene|389245FDE13226D65DF117E28B5DA346DC856860|msrA]-[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]
    description
    [Pubmed|18662407]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|18662407,17289755], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx]: activation, oxidized [protein|2C6386E9A63F410558D168798D077DF91590F454|Spx] binds [protein|5996A5C25E108A3C4562686BF34A59CB14FD56EB|RpoA] and enhances [protein|389245FDE13226D65DF117E28B5DA346DC856860|MsrA]-[protein|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|MsrB] operon transcription [Pubmed|18662407], in [regulon|2C6386E9A63F410558D168798D077DF91590F454|Spx regulon]

    • regulation

  • induced by oxidative stress ([protein|search|Spx])
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    Biological materials

    Mutant

  • MGNA-A905 (yppQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/905 NBRP B. subtilis, Japan]
  • BKE21680 (Δ[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE21680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTCTTCTTTATTGTACG, downstream forward: _UP4_TAAACTGTTAAAAACAGGCC
  • BKK21680 (Δ[gene|BF8A3C916C4B673D30AEF7C330C9BD5A487AB0EA|msrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK21680 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTTCTTCTTTATTGTACG, downstream forward: _UP4_TAAACTGTTAAAAACAGGCC

    • References

  • 18662407